This is a feed that looks for Oxford Nanopore Technologies (not affiliated) DNA, RNA, and/or Protein Sequencing related posts based on text with nanopore or oxford nanopore. Does not look for ont. // Made at BlueskyFeeds.com
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www.biorxiv.org
Single-molecule nanopore sequencing reveals spatial coordination of rRNA modifications in human ribosomes
Ribosomal RNA has a high density of epitranscriptomic modifications essential for faithful translation, and their levels vary across cell types and in disease. Typically, rRNA modifications are quanti...
www.nature.com
A versatile nanopore identifies four classes of analytes simultaneously - Nature Biotechnology
A versatile nanopore sensor, created by modifying a bacterial porin, enables the simultaneous identification of amino acids, nucleoside monophosphates, saccharides and peptides. Coupled with machine learning, it achieves 98.7% classification accuracy and enables the compositional analysis of native glycopeptides.
dlvr.it
Oxford Nanopore wins injunction against MGI Tech in Europe
The Unified Patent Court barred MGI Tech from selling its CycloneSEQ sequencing systems across eight European jurisdictions after ruling they infringe two Oxford Nanopore patents.
www.nature.com
Nanopore sequencing identifies parent-of-origin specific age-associated methylation changes at imprinted loci in the human genome - Nature Communications
DNA methylation changes with age, but parent-of-origin effects remain poorly characterised. Here, the authors use nanopore sequencing to map age-associated methylation across the genome, revealing wid...
www.nature.com
An engineered nanopore identifies saccharides, amino acids, peptides and ribonucleotides
(a) Sensing of phenylalanine (Phe) with MspA-PBA. Left: The structure of MspA-PBA. Right: A representative trace of Phe sensing performed with MspA-PBA. (b) Sensing of D-fructose (Fru) with MspA-NTA-Ni. Left: The structure of MspA-NTA-Ni. Right: A representative trace of Fru sensing performed with MspA-NTA-Ni. (c) Sensing of guanosine 5’-monophosphate (GMP) with MspA-NTA-Ni. Left: The structure of MspA-NTA-Ni. Right: A representative trace of GMP sensing performed with MspA-NTA-Ni. The final concentrations of Phe, Fru and GMP were 400 μM, 20 mM and 2 mM, respectively. No nanopore events were observed in all above measurements. The open pore current of MspA-PBA ( + 160 mV bias) and MspA-NTA-Ni (+100 mV bias) were defined as IPBA and INTA-Ni, respectively. The corresponding open pore current was marked by a gray dashed line. (a) Representative events of different amino acids, NMPs and saccharides acquired by MspA-FPBA (Methods). 21 amino acids (including 20 common proteinogenic amino acids and selenocysteine), 4 NMPs (adenosine 5’-monophosphate, AMP; uridine 5’-monophosphate, UMP; cytidine 5’-monophosphate, CMP; guanosine 5’-monophosphate, GMP), and 4 saccharides (iduronic acid, IdoA; L-arabinose, Ara; D-fructose, Fru; N-acetyl-D-glucosamine, GlcNAc) were respectively added to the cis side during the measurement. Cysteine, histidine and lysine each have two representative types of events,...
www.nature.com
An engineered nanopore identifies saccharides, amino acids, peptides and ribonucleotides - Nature Biotechnology
Modified nanopore simultaneously identifies diverse biomolecules and their modifications.
Jean P. Elbers 
